| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Kaplan, Nathan X (9)
|
MRYFD |
36.16 |
35.11 |
-1.05 |
| 3 |
Shcherban, Dmytro (9)
|
MRCOND |
36.91 |
35.25 |
-1.66 |
| 4 |
Yi, Aiden L (9)
|
NJJFAC |
38.96 |
37.28 |
-1.68 |
| 5 |
Zagorski, Sebastian J (9)
|
MRRA |
37.73 |
39.40 |
1.67 |
| 6 |
Zhang, Nicolas C (9)
|
MRBGNW |
38.68 |
40.09 |
1.41 |
| 7 |
Rogerson, Ethan V (9)
|
MRBGNW |
39.72 |
40.87 |
1.15 |
| 8 |
Winnegar, Carter J (9)
|
MRBGNW |
47.09 |
40.98 |
-6.11 |
| 9 |
Rybak, Peter M (9)
|
CTZEUS |
49.20 |
43.05 |
-6.15 |
| 10 |
van der Touw, Samuel B (9)
|
NJJFAC |
43.87 |
45.07 |
1.20 |
| 11 |
Bajgierowicz, Michael (9)
|
MRCOND |
47.10 |
45.68 |
-1.42 |
| 12 |
Villanueva, Michael (9)
|
CTZEUS |
|
46.26 |
- |
| 13 |
Saloutenko, Hunter H (9)
|
MRCOND |
53.84 |
49.63 |
-4.21 |